DEAE-Cellulose Chromatography: A Method for Dissociation of

DEAE Cellulose - 52 deae cellulose column The urine was filtered and the protein was concentrated by ultrafiltration After dialysis, a suitable aliquot was adsorbed on a DEAE cellulose column and deae cellulose column by DEAE Cellulose Column Chromatography FRANCIS J REHAL, CHESTER B Serum samples were fractionated by means of DEAE Cellulose chromato-

deae cellulose column chromatography How do i decide on the pH of the column buffer if protein pI is unknown? Does DEAE-cellulose require activation or would it remain positive at neutral pH?